High Performance Dichroic Filters for Fluorescence Microscopy
  • Patented Stabilife® Hard Coatings
  • Lowest autofluorescence
  • Maximum transmission
  • Optimized optical fabrication for brilliant imaging
  • Superior field life

Product Description

Corion High Performance Fluorescence (HPF™) dichroic filters for fluorescence microscopy have been manufactured to provide maximum performance using several key design features including exceptionally high transmission in the transmission band, maximized reflectance in the reflectance band, high-precision optical glass substrates, and spectral specifications providing maximum image brightness and minimum background.

Our HPF™ filter offering is a compilation of products based on designs that were developed to be used in fluorescence-based instrumentation including DNA Sequencers, Cytometers, Microplate Readers, In vivo Imagers, and Thermocyclers, offered by market-leading biotech instrument manufacturers. We are now making these OEM-proven designs available to the individual researchers through our catalog offering of High Performance Filters for fluorescence microscopy. HPF™ filters are manufactured using our patented Stabilife® coating technology which provides excellent spectral performance, spectral stability, and physical durability.

The individual filters were originally designed as elements of a set, typically containing an excitation filter, a dichroic filter, and an emission filter. As such, particular emphasis was placed on reducing crosstalk between the excitation and emission channels corresponding to the absorbance and emission bands of the particular fluorophore/s for which the set was designed. The dichroic filters have been optimized to provide the best performance from the set.

Custom Products and Services

Custom sizes of standard HPF™ filters and filter sets as well as mounting services are available upon request. In addition to our offering of standard fluorescence filters, we manufacture custom fluorescence filters as OEM components for many market-leading producers of biotechnology instruments for both clinical and research applications. To discuss your specific requirements, please call 508-528-4411 to speak directly to one of our Technical Sales Engineers, or email us at filters.sales@newport.com.

Trademark Information

Alexa Fluor®, BODIPY®, Calcium Crimson™, LysoSensor™, MitoTracker®, Oregon Green®, Pacific Blue™, Rhodamine Green™, and Texas Red® are trademarks of Molecular Probes, Inc.

Cy is a trademark of GE Healthcare, Ltd.

Stabilife® and HPF™ are trademarks of Newport Corporation


Ordering Information

ModelTransmission Band
(Tavg: Range)
Reflectance Band
(Ravg: Range)
Filter SizeFilter Thickness
HPD400%T ≥ 93%: 425 - 515nm%R ≥ 95%: 325 - 395nm25.5 x 36.0mm1.0mm
HPD410%T ≥ 93%: 425 - 515nm%R ≥ 95%: 325 - 395nm25.5 x 36.0mm1.0mm
HPD440%T ≥ 93%: 454 - 495nm%R ≥ 95%: 375 - 420nm25.5 x 36.0mm1.0mm
HPD455%T ≥ 93%: 492 - 510nm%R ≥ 95%: 420 - 425nm25.5 x 36.0mm1.0mm
HPD480%T ≥ 93%: 487 - 545nm%R ≥ 95%: 420 - 475nm25.5 x 36.0mm1.0mm
HPD490%T ≥ 93%: 520 - 570nm%R ≥ 95%: 400 - 445nm25.5 x 36.0mm1.0mm
HPD495%T ≥ 93%: 512 - 570nm%R ≥ 95%: 440 - 492nm25.5 x 36.0mm1.0mm
HPD505%T ≥ 93%: 512 - 570nm%R ≥ 95%: 455 - 500nm25.5 x 36.0mm1.0mm
HPD515%T ≥ 93%: 520 - 565nm%R ≥ 95%: 460 - 510nm25.5 x 36.0mm1.0mm
HPD520%T ≥ 93%: 531 - 750nm%R ≥ 95%: 480 - 511nm25.5 x 36.0mm1.0mm
HPD525%T ≥ 93%: 530 - 595nm%R ≥ 95%: 470 - 523nm25.5 x 36.0mm1.0mm
HPD530%T ≥ 93%: 537 - 610nm%R ≥ 95%: 470 - 523nm25.5 x 36.0mm1.0mm
HPD540%T ≥ 93%: 550 - 615nm%R ≥ 95%: 480 - 532nm25.5 x 36.0mm1.0mm
HPD550%T ≥ 93%: 567 - 620nm%R ≥ 95%: 490 - 550nm25.5 x 36.0mm1.0mm
HPD555%T ≥ 93%: 567 - 620nm%R ≥ 95%: 490 - 550nm25.5 x 36.0mm1.0mm
HPD565%T ≥ 93%: 587 - 650nm%R ≥ 95%: 500 - 565nm25.5 x 36.0mm1.0mm
HPD570%T ≥ 93%: 587 - 650nm%R ≥ 95%: 500 - 565nm25.5 x 36.0mm1.0mm
HPD580%T ≥ 93%: 592 - 660nm%R ≥ 95%: 540 - 582nm25.5 x 36.0mm1.0mm
HPD585%T ≥ 93%: 592 - 660nm%R ≥ 95%: 540 - 582nm25.5 x 36.0mm1.0mm
HPD590%T ≥ 93%: 612 - 675nm%R ≥ 95%: 532 - 585nm25.5 x 36.0mm1.0mm
HPD595%T ≥ 93%: 608 - 700nm%R ≥ 95%: 525 - 588nm25.5 x 36.0mm1.0mm
HPD610%T ≥ 93%: 619 - 680nm%R ≥ 95%: 540 - 590nm25.5 x 36.0mm1.0mm
HPD635%T ≥ 93%: 647 - 710nm%R ≥ 95%: 575 - 626nm25.5 x 36.0mm1.0mm
HPD645%T ≥ 93%: 620 - 720nm%R ≥ 95%: 570 - 646nm25.5 x 36.0mm1.0mm
HPD655%T ≥ 93%: 667 - 725nm%R ≥ 95%: 585 - 650nm25.5 x 36.0mm1.0mm
HPD660%T ≥ 93%: 673 - 740nm%R ≥ 95%: 600 - 652nm25.5 x 36.0mm1.0mm
HPD675%T ≥ 93%: 686 - 745nm%R ≥ 95%: 615 - 669nm25.5 x 36.0mm1.0mm
HPD680%T ≥ 93%: 692 - 760nm%R ≥ 95%: 590 - 667nm25.5 x 36.0mm1.0mm
HPD695%T ≥ 93%: 705 - 780nm%R ≥ 95%: 630 - 684nm25.5 x 36.0mm1.0mm
HPD755%T ≥ 93%: 765 - 860nm%R ≥ 95%: 675 - 737nm25.5 x 36.0mm1.0mm
HPD760%T ≥ 93%: 765 - 860nm%R ≥ 95%: 675 - 737nm25.5 x 36.0mm1.0mm

Specifications

Transmittance Band Transmittance≥90%
Reflectance Band Reflectance≥90%
Surface QualityE/E (60/40) per MIL-F-48616 (typical)
Coating HardnessMIL-C-48497
Coating Abrasion ResistanceMIL-C-48497
Coating AdhesionMIL-C-48497
Coating Humidity ResistanceMIL-STD-810, Method 507.3, Procedure III, Modified to 40 cycles
Coating Operating Temperature Range-100 °C to 300 °C
Filter Size25.5 x 36 mm
Filter Thickness1.0 mm

Fluorophore Reference - HPF Dichroic Filters

FLUOROPHOREDICHROIC
5-FAMHPD505
5-ROXHPD590
5-TAMRAHPD560
6-HEXHPD550
6-JOEHPD515
6-TETHPD530
Alexa Fluor 350HPD400
Alexa Fluor 430HPD490
Alexa Fluor 488HPD505
Alexa Fluor 532HPD530
Alexa Fluor 546HPD560
Alexa Fluor 555HPD560
Alexa Fluor 568HPD595
Alexa Fluor 594HPD595
Alexa Fluor 633HPD645
Alexa Fluor 647HPD660
Alexa Fluor 660HPD680
Alexa Fluor 680HPD695
Alexa Fluor 700HPD695
Alexa Fluor 750HPD760
AMCAHPD400
BODIPY 630/650HPD635
BODIPY 650/665HPD660
BODIPY FLHPD505
BODIPY R6GHPD540
BODIPY TMRHPD560
BODIPY TRHPD610
Calcium Crimson™HPD595
CFPHPD455
Cy3.5™HPD580
Cy3™HPD565
Cy5.5™HPD675
Cy5™HPD655
Cy7™HPD755
DAPIHPD400
Dil(rs)HPD565
DiOHPD505
DsRedHPD570
EosinHPD525
Ethidium BromideHPD565
FITCHPD505
Fluo 3HPD505
Fluo 4HPD505
GFPHPD495
GFP(rs)HPD505
GFP(wt)HPD480
Hoechst 33342 & 33258HPD400
LysoSensor™ Blue (pH 5)HPD410
MitoTracker OrangeHPD560
MitoTracker RedHPD590
Nile RedHPD565
Oregon Green™HPD515
Pacific Blue™HPD440
PE (B,R)HPD560
Propidium IodideHPD565
ResorufinHPD595
RhodamineHPD585
Rhodamine Green™HPD515
Texas RedHPD595
TRITCHPD560
YFPHPD525
YGFPHPD515