Fluorescence Filters – Custom Specified

Product Description

Fluorescence filters are technically not a true filter type like bandpass or dichroic, but rather, this category includes true filter types such as bandpass and dichroic that have been optimized for fluorescence detection. Many attributes must be considered when designing fluorescence filters such as selection of material to minimize autofluorescence, whether or not the filter will be used for imaging, and placement of excitation and emission bands to deliver sufficient excitation energy to the sample and collect the maximum amount of fluorescence emission while minimizing crosstalk between the two channels. Emission signals are often very low in amplitude so maximizing the signal-to-noise ratio is often the most important parameter to consider for optimum performance in an instrument.

The majority of the fluorescence filters that we manufacture are custom designed for OEM instrumentation such as DNA Analyzers, Flow Cytometers, PCR Thermocyclers, Microplate Readers, and many more. Working in partnership with the instrument manufacturer's optical engineers and biotech scientists, we design custom excitation and emission bandpass filters, and in some cases, dichroic filters, that are optimized for the specific fluorophores that will be used in the instrument while taking into consideration all of the nuances of the instrument architecture and the assays to be performed on it. This team approach enables delivery of the best cost / performance / time-to-market value to the instrument maker.

We make fluorescence bandpass filters in several different configurations:

Single substrate, image quality, all-dielectric hard sputtered coating

Single substrate, general quality, all-dielectric hard sputtered coating

Multi-element, image quality, all-dielectric hard sputtered coating, laminated assembly

Multi-element, general quality, all-dielectric hard sputtered coating, laminated assembly

Multi-element, image quality, semi-hard thermally evaporated coating, laminated assembly

All of these configurations can deliver exceptional performance at economical cost. For many applications, the single substrate configuration is the best choice, but for some of the most demanding applications where the absolute best signal-to-noise ratio possible is necessary, our multi-element - laminated assembly configuration is the only choice that will guarantee optimum signal-to-noise ratio. We recommend the best configuration given the requirements of the instrument.

Our dichroic filters for fluorescence applications and all manufactured using our single substrate, image quality, all-dielectric hard sputtered coating configuration. Special attention is focused during design to insure minimized wavefront distortion and image shift.

Fluorescence Filter Specification Checklist

The list of parameters that follows includes the most common items used to specify a fluorescence bandpass or dichroic filter. Many of the parameters are general enough to apply to any optical filter. Care should always be taken to avoid over-specification. For example, specifying some of the specifications in the optical category such as transmitted wavefront error can, depending on the chosen value, have a significant impact on cost while providing no increase in filter performance if the filter is used in a photometric detection application. When using this checklist, the best strategy is to provide what is known to be important and allow our technical team to recommend other specifications from the list that will insure the best performance for the application. After completing the checklist, please call 508-528-4411 to speak directly to one of our Technical Sales Engineers, or email us at filters.sales@newport.com to address your specific requirements.

Fluorescence Filter Specification Checklist

Application
Fluorophore/s
Type (Imaging Emission, Photometric Emission, Excitation, Dichroic)
Paired Usage (Y/N)
 
Operating Conditions
Operating Temperature
Storage Temperature
Angle of Incidence
System Speed
Detector
Illumination Source
Polarization State
 
 
Physical
Length / Length tolerance
Width / Width tolerance
Thickness / Thickness tolerance (or max)
Active Area (min)
Edge treatment-Bevel
Edge treatment-Ring
 
 
Optical
Surface Quality
Flatness (S1)
Flatness (S2)
Parallelism
 
 
Durability
Hardness
Adhesion
Humidity
 
 
Spectral (Bandpass)
Center wavelength
Bandwidth (FWHM)
Low half-power wavelength
High half-power wavelength
%Tpk
%Tavg
%Tavg range
Edge Steepness
BW@ 10% Tpk
BW@ 1.0% Tpk
BW@ 0.1% Tpk
BW@ 0.01% Tpk
BW@ 0.001% Tpk
Blocking range
Blocking (min OD / max %T over range)
Blocking, Integrated (S/N) Range of Transmittance
 
 
Spectral (Dichroic)
Transmittance band
%Tavg in transmittance band
Reflectance band
%Ravg in reflectance band
Cut-on wavelength (nominal @ 50%Tpk)
 
 
Other