High Performance Filters for Fluorescence Microscopy
  • Patented Stabilife® Coatings
  • Shift free spectral response @ 0% - 100%RH
  • Humidity per MIL-STD-810, Method 507.3, Procedure III, 20 cycles
  • Lowest autofluorescence
  • Extremely sharp transitions from rejection to transmission regions
  • Maximum transmission
  • Excellent set signal-to-noise ratio
  • Optimized optical fabrication for brilliant imaging
  • Superior field life

Product Description

HPF™ filter sets for fluorescence microscopy have been manufactured to provide maximum performance using several key design features including exceptionally high passband transmission, maximized out-of-band blocking including extended range blocking on excitation filters, extremely steep slopes from rejection to transmission regions, high-precision optical glass substrates, and spectral specifications providing maximum image brightness and minimum background.

Corion HPF™ series of filters for fluorescence detection is a compilation of product designs of filters that was developed to be used with many of the most popular standard fluorophores and some of the most promising new fluorophores. Since their introduction, these product designs have served as the starting point for custom designed filters for market leading instruments including DNA Sequencers, Cytometers, Microplate Readers, In vivo Imagers, and Thermocyclers. We are now making these OEM proven designs available to the individual researcher through our catalog offering of High Performance Filter sets for fluorescence microscopy. HPF™ filters are manufactured using Newport's patented Stabilife® coating technology which provides excellent spectral performance, spectral stability, and physical durability.

Filter Set Selection

HPF™ Fluorescence Filter Sets are listed in two tables. The Fluorophore Reference table lists the recommended filter set for each listed fluorophore. The Numerical Reference table identifies all of the fluorophores that can be utilized with each filter set. Spectral performance of each filter set is provided for reference in a series of data curves following the tables.

Individual Fluorescence Filters

All individual excitation, dichroic, and emission filters included in our filter set offering can be purchased separately.

Custom Products and Services

Custom sizes of standard HPF™ filters and filter sets as well as mounting services are available upon request. In addition to our offering of standard fluorescence filters, we manufacture custom fluorescence filters as OEM components for many market-leading producers of biotechnology instruments for both clinical and research applications. To discuss your specific requirements, please call 508-528-4411 to speak directly to one of our Technical Sales Engineers, or email us at filters.sales@newport.com.


Ordering Information

HPF™ Fluorescence Filter Sets - Fluorophore Reference
FluorophoreFilterSetExcitation FilterDichroic FilterEmission Filter
5-FAMHPF1245HPX475-44HPD505HPM535-50
5-ROXHPF1340HPX560-50HPD590HPM640-60
5-TAMRAHPF1335HPX525-45HPD560HPM620-75
6-HEXHPF1295HPX520-40HPD550HPM590-55
6-JOEHPF1230HPX500-20HPD515HPM530LP
6-TETHPF1280HPX500-40HPD530HPM575-50
Alexa Fluor 350HPF1205HPX360-60HPD400HPM460-60
Alexa Fluor 430HPF1255HPX425-40HPD490HPM540-40
Alexa Fluor 488HPF1245HPX475-44HPD505HPM535-50
Alexa Fluor 532HPF1275HPX500-40HPD530HPM560-50
Alexa Fluor 546HPF1300HPX525-45HPD560HPM600-60
Alexa Fluor 555HPF1300HPX525-45HPD560HPM600-60
Alexa Fluor 568HPF1345HPX560-40HPD595HPM640-60
Alexa Fluor 594HPF1345HPX560-40HPD595HPM640-60
Alexa Fluor 633HPF1385HPX610-60HPD645HPM685-50
Alexa Fluor 647HPF1400HPX620-50HPD660HPM700-60
Alexa Fluor 660HPF1410HPX630-60HPD680HPM720-60
Alexa Fluor 680HPF1415HPX660-40HPD695HPM725-40
Alexa Fluor 700HPF1420HPX660-40HPD695HPM740-50
Alexa Fluor 750HPF1430HPX710-50HPD760HPM810-80
AMCAHPF1205HPX360-60HPD400HPM460-60
BODIPY 630/650HPF1380HPX605-40HPD635HPM670-50
BODIPY 650/665HPF1395HPX630-40HPD660HPM695-50
BODIPY FLHPF1250HPX480-40HPD505HPM535-50
BODIPY R6GHPF1285HPX510-40HPD540HPM575-50
BODIPY TMRHPF1305HPX530-40HPD560HPM600-60
BODIPY TRHPF1355HPX570-40HPD610HPM645-50
Calcium CrimsonHPF1365HPX570-40HPD595HPM645-60
CFPHPF1220HPX440-20HPD455HPM480-40
Cy3.5™HPF1325HPX565-30HPD580HPM620-60
Cy3™HPF1310HPX535-40HPD565HPM610-50
Cy5.5™HPF1405HPX645-40HPD675HPM710-50
Cy5™HPF1390HPX620-50HPD655HPM690-50
Cy7™HPF1425HPX710-50HPD755HPM800-70
DAPIHPF1205HPX360-60HPD400HPM460-60
Dil(rs)HPF1315HPX535-50HPD565HPM620-60
DiOHPF1250HPX480-40HPD505HPM535-50
DsRedHPF1320HPX545-30HPD570HPM620-60
EosinHPF1270HPX500-40HPD525HPM560-50
Ethidium BromideHPF1350HPX525-45HPD565HPM640-70
FITCHPF1250HPX480-40HPD505HPM535-50
Fluo 3HPF1250HPX480-40HPD505HPM535-50
Fluo 4HPF1250HPX480-40HPD505HPM535-50
GFPHPF1240HPX470-40HPD495HPM535-50
GFP(rs)HPF1250HPX480-40HPD505HPM535-50
GFP(wt)HPF1225HPX450-50HPD480HPM510-50
Hoechst 33342 & 33258HPF1200HPX350-60HPD400HPM460-60
LysoSensor™ Blue (pH 5)HPF1210HPX380-40HPD410HPM460-60
MitoTracker OrangeHPF1335HPX525-45HPD560HPM620-75
MitoTracker RedHPF1340HPX560-50HPD590HPM640-60
Nile RedHPF1360HPX525-45HPD565HPM645-60
Oregon Green™HPF1260HPX485-30HPD515HPM545-30
Pacific Blue™HPF1215HPX400-30HPD440HPM470-30
PE (B,R)HPF1290HPX525-45HPD560HPM585-40
Propidium IodideHPF1370HPX525-45HPD565HPM645-75
ResorufinHPF1375HPX560-50HPD595HPM645-75
RhodamineHPF1330HPX570-20HPD585HPM620-60
Rhodamine GreenHPF1235HPX500-20HPD515HPM535-30
Texas RedHPF1375HPX560-50HPD595HPM645-75
TRITCHPF1300HPX525-45HPD560HPM600-60
YFPHPF1265HPX500-20HPD525HPM550-40
YGFPHPF1235HPX500-20HPD515HPM535-30

HPF™ Fluorescence Filter Sets - Numerical Reference (set number)
FluorophoreFilterSetExcitation FilterDichroic FilterEmission Filter
Hoechst 33342 & 33258HPF1200HPX350-60HPD400HPM460-60
Alexa Fluor 350, AMCA, DAPIHPF1205HPX360-60HPD400HPM460-60
LysoSensor™ Blue (pH 5)HPF1210HPX380-40HPD410HPM460-60
Pacific Blue™HPF1215HPX400-30HPD440HPM470-30
CFPHPF1220HPX440-20HPD455HPM480-40
GFP(wt)HPF1225HPX450-50HPD480HPM510-50
6-JOEHPF1230HPX500-20HPD515HPM530LP
Rhodamine Green , YGFPHPF1235HPX500-20HPD515HPM535-30
GFPHPF1240HPX470-40HPD495HPM535-50
5-FAM, Alexa Fluor 488HPF1245HPX475-44HPD505HPM535-50
BODIPY FL, DiO, FITC, Fluo 3, Fluo 4, GFP(rs)HPF1250HPX480-40HPD505HPM535-50
Alexa Fluor 430HPF1255HPX425-40HPD490HPM540-40
Oregon Green™HPF1260HPX485-30HPD515HPM545-30
YFPHPF1265HPX500-20HPD525HPM550-40
EosinHPF1270HPX500-40HPD525HPM560-50
Alexa Fluor 532HPF1275HPX500-40HPD530HPM560-50
6-TETHPF1280HPX500-40HPD530HPM575-50
BODIPY R6GHPF1285HPX510-40HPD540HPM575-50
PE (B,R)HPF1290HPX525-45HPD560HPM585-40
6-HEXHPF1295HPX520-40HPD550HPM590-55
Alexa Fluor 546, Alexa Fluor 555, TRITCHPF1300HPX525-45HPD560HPM600-60
BODIPY TMRHPF1305HPX530-40HPD560HPM600-60
Cy3™HPF1310HPX535-40HPD565HPM610-50
Dil(rs)HPF1315HPX535-50HPD565HPM620-60
DsRedHPF1320HPX545-30HPD570HPM620-60
Cy3.5™HPF1325HPX565-30HPD580HPM620-60
RhodamineHPF1330HPX570-20HPD585HPM620-60
5-TAMRA, MitoTracker OrangeHPF1335HPX525-45HPD560HPM620-75
5-ROX, MitoTracker RedHPF1340HPX560-50HPD590HPM640-60
Alexa Fluor 568, Alexa Fluor 594HPF1345HPX560-40HPD595HPM640-60
Ethidium BromideHPF1350HPX525-45HPD565HPM640-70
BODIPY TRHPF1355HPX570-40HPD610HPM645-50
Nile RedHPF1360HPX525-45HPD565HPM645-60
Calcium CrimsonHPF1365HPX570-40HPD595HPM645-60
Propidium IodideHPF1370HPX525-45HPD565HPM645-75
Resorufin, Texas RedHPF1375HPX560-50HPD595HPM645-75
BODIPY 630/650HPF1380HPX605-40HPD635HPM670-50
Alexa Fluor 633HPF1385HPX610-60HPD645HPM685-50
Cy5™HPF1390HPX620-50HPD655HPM690-50
BODIPY 650/665HPF1395HPX630-40HPD660HPM695-50
Alexa Fluor 647HPF1400HPX620-50HPD660HPM700-60
Cy5.5™HPF1405HPX645-40HPD675HPM710-50
Alexa Fluor 660HPF1410HPX630-60HPD680HPM720-60
Alexa Fluor 680HPF1415HPX660-40HPD695HPM725-40
Alexa Fluor 700HPF1420HPX660-40HPD695HPM740-50
Cy7™HPF1425HPX710-50HPD755HPM800-70
Alexa Fluor 750HPF1430HPX710-50HPD760HPM810-80

Specifications

 Excitation FiltersDichroic FiltersEmission Filters
Passband Transmittance≥90%≥90%≥90%
Dichroic ReflectanceN/A≥90%N/A
Spectral BlockingOD6N/AOD6
Surface QualityE/E (60/40) per MIL-F-48616 (typical)E/E (60/40) per MIL-F-48616 (typical)E/E (60/40) per MIL-F-48616 (typical)
Coating HardnessMIL-C-48497MIL-C-48497MIL-C-48497
Coating Abrasion ResistanceMIL-C-48497MIL-C-48497MIL-C-48497
Coating AdhesionMIL-C-48497MIL-C-48497MIL-C-48497
Coating Humidity ResistanceMIL-STD-810, Method 507.3, Procedure III, Modified to 40 cycles**MIL-STD-810, Method 507.3, Procedure III, Modified to 40 cycles**MIL-STD-810, Method 507.3, Procedure III, Modified to 40 cycles**
Coating Operating Temperature Range-100 °C to 300°C**-100 °C to 300°C**-100 °C to 300°C**
Filter Size25 mm dia.25.5 mm x 36 mm25 mm dia.
Filter Thickness5 mm1 mm5 mm

**The specifications for humidity resistance and coating operating temperature range listed above apply to exposed coatings only. Humidity resistance and operating temperature range of filters manufactured using Stabilife® coatings and assembled using epoxy systems revert to the humidity resistance and operating temperature range of the epoxy system.


Trademark Information

Alexa Fluor®, BODIPY®, Calcium Crimson™, LysoSensor™, MitoTracker®, Oregon Green®, Pacific Blue™, Rhodamine Green™, and Texas Red® are trademarks of Molecular Probes, Inc.

Cy is a trademark of GE Healthcare, Ltd.

Stabilife® and HPF™ are trademarks of Newport Corporation